GLOBAL NETWORK OF BANGLADESHI BIOTECHNOLOGISTS

CRISPR-Cas9 Genome Editing Technology

Venue

Centre for Advanced Research in Sciences (CARS), Conference room, University of Dhaka, Dhaka 1000, Bangladesh

Speaker's Detail

  • Dr. Abidur Rahman

  • (MSc. PhD) 
    Associate Professor 
    Department of Plant Bio-Sciences
    Faculty of Agriculture
    Iwate University
    Japan 

  • Dr. Mohammad Aslam

  • (MSc. PhD) 
    Japan Society for Promotion of Science
    (JSPS) Postdoctoral Fellow
    Department of Plant Bio Sciences
    Faculty of Agriculture Iwate University
    Japan 

Target Institutions

  • Research institutions
  • Universities
  • Private Industries

Registration

  • Universities, Research Institutions

  • 1000 Tk

  • Private Companies

  • 5000 Tk
  • GNOBB, Sonali Bank, DU branch; 
    Acc no: 44-057-3301-4308
  • Please send Email to Dr. khademul Islam: workshop@gnobb.org

Refreshment Venue

CARS Cafeteria

Group Size

30

Target people

Early career researchers and scientists preferably working in plant science

Eligibility criteria

Basic knowledge on Molecular biology. Institutions will be requested to nominate two people with a short CV, describing their experience on plant sciences. Selected candidates will be notified.

Special instruction

Participants will be instructed to bring their Laptop with WiFi receiver

Workshop Plan

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Time Session Duration
Registration 30 Minutes 
Inaugural Session 30 Minutes 
Welcome address by President GNOBB, Professor Dr Zeba I Seraj, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh 5 Minutes 
CRISPR‐Cas9 technology and its application in Genome Editing, Dr. Abidur Rahman, Associate Professor, Department of Plant Bio‐ Sciences, Faculty of Agriculture, Iwate University, Ueda 3‐18‐8, Morioka, Iwate, Morioka 0208550, Japan. 10 Minutes 
Address by Special Guest, Professor Dr M Imdadul Hoque, Dean, Faculty of Biological Sciences, University of Dhaka, Bangladesh 5 Minutes 
Address by Special Guest, Dr Kazi M Kamaruddin, Acting Executive Director of Krishi Gobeshona Foundation 5 Minutes 
Address by Chief Guest, Professor Dr AAMS Arefin Siddique, Honorable Vice Chancellor, University of Dhaka, Bangladesh 5 Minutes 
Vote of thanks by Head of Education Section, GNOBB 5 Minutes 
Tea break 25 Minutes 
Technical Session 6 Hours
Introduction of CRISPR technologies: Cas9 protein and gRNAs 30 Minutes 
The principle to select target sequences to design gRNA sequences 30 Minutes 
Synthesize gRNA sequences and selecting vectors: a. outsource strategy (IDT Singapore) b. Synthesize gRNAs by PCR   c. Source for vectors  1 Hour
Golden gate cloning: An introduction 1 Hour
Lunch and prayer break 1 Hour
Genome editing in Arabidopsis‐ A case study in our lab. Brief idea about rice transformation, screening for plants with evidence of mutation 1 Hour
Knowledge assessment: Design your gRNAs with your favorite genes 1 Hour
Biosafety regulation on GMO in Bangladesh 10 Minutes 
CRISPR/Cas9 and Biosafety regulation 15 Minutes 
Distribution of ‘Certificate of participation’ 10 Minutes 
Concluding remarks by General Secretary, GNOBB 10 Minutes 
Photo session, Tea and End 15 Minutes 

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