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Agrobacterium-mediated transformation of soybean cultivar with PR 10a from the potato cultivar Desiree

Agrobacterium-mediated transformation of soybean cultivar with PR 10a from the potato cultivar Desiree

In an article entitled, “Enhanced tolerance to drought and salt stresses in transgenic faba bean (Vicia faba L.) plants by heterologous expression of the PR10a gene from potato,” published in the March issue of Plant Cell Reports, the lead author Moemen S. Hanafy, and four other |associates under the guidance of Professor Dr. H-J Jacobsen at the Institute of Plant Genetics, Hannover, Germany report for the first time the expression of PR10a gene from the potato cultivar Desiree to soybean cultivar Tattoo. The authors used a well-known protocol, namely, Agrobacterium tumefaciens mediated transformation. The transgenic soy variety plants showed enhanced tolerance to both drought and salinity conferred by PR10a gene characterizing the potato cultivar Desiree. The authors confirmed the incorporation of transgene by testing the putative individuals by PCR,  RT-PCR, Southern blot and monitoring of Luciferase activity. They obtained further proof of the incorporation of PR10a gene by growing the putative transgenics under drought condition after withholding water for 3 weeks. The leaves of  transgenic plants were still green, while non-transgenic plants (WT) wilted and turned brown. Moreover, the transgenic lines displayed higher tolerance to NaCl stress. Their results suggested that introducing a novel PR10a gene into faba bean could be a promising approach to improve its drought and salt tolerance ability, and that MAS promoter is not only constitutive, but also wound-, auxin/cytokinin- as well as stress-inducible.

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