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“Biotransformation of low cost lignocellulosic substrates into vanillin by white rot fungus, Phanerochaete chrysosporium

“Biotransformation of low cost lignocellulosic substrates into vanillin by white rot fungus, Phanerochaete chrysosporium

In an article entitled, “Biotransformation of low cost lignocellulosic substrates into vanillin by white rot fungus, Phanerochaete chrysosporium,” published in the April issue Indian J. Biotech. 12: 281-283, Karode Bhusan, Patil Ulhas and Aparna Jobanputra have reported that they used lignocellulosic substrates such as rice husk, groundnut shell, etc. to transform  into vanillin (4-hydroxy-3-methoxybenzaldehyde), a pleasant smelling aromatic compound. It may be mentioned here that vanilla occurs naturally in vanilla beans. By using a white rot fungus, namely, Phanerochaete chrysosporium NCIM 1197, the above authors succeeded in obtaining vanilla indicating it’s possible to degrade  cheap lignocellulosic substrates to vanilla. The method described here opens up the possibility of of obtaining vanilla by a cheap method, namely, by degrading groundnut shell. They confirmed that lignocellulosic substrates like rice husk, groundnut shell, etc. can be used for biodegradation. Further, detection and quantification of vanillin were carried out by TLC and spectrophotometrically. The maximum vanillin production (up to 55 µg/mL) was observed within 72 h using groundnut shell as a substrate. The use of various carbon sources, viz., glucose and starch, along with groundnut shell, affected vanillin production. Supplementation of glucose along with groundnut shell showed more increase in vanillin production (37 µg/mL within 96 h) in comparison to the supplementation of starch (up to 21 µg/mL within 96 h).

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