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Construction of a genetic map of carrot pinpointing Genes controlling vernalization and male sterility

Construction of a genetic map of carrot pinpointing Genes controlling vernalization and male sterility

In an article entitled, “Molecular mapping of vernalization requirement and fertility   restoration genes in carrot,” published in Sept. 26, 2012 issue of TAG Theoretical  and Applied Genetics (vol 125, 2012) MS Alessandro and three of her associates reported the genes responsible for vernalization. Carrot (Daucus carota 2n = 18) is characterized by both annual and biennial varieties, the first-mentioned ones are suited tropical climate not requiring rigorous vernilization.  They found one major gene controlling floral initiation and several male-fertility restoring genes. For the first time the authors developed  the linkage map of the genomic regions that control vernalization response and fertility restoration. In order to prepare the linkage map they used an F2 progeny from the cross, annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot. They named the two single dominant genes  controlling early flowering habit and restoration of petaloid cytoplasmic male sterility as  Vrn1 and Rf1, respectively. They prepared a map of all the nine chromosomes consisting of 355 markers with a total map length of 669 cM. Furthermore, they determined the average marker-to-marker distance of 1.88 cM. They also identified flanking markers of both the dominant genes. Identification of  marker genes and their locations will provided important tools to carrot breeders to evolve varieties with desirable traits.

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