In vitro flowering and seed formation in lentil

In vitro flowering and seed formation in lentil

In  an article, “In vitro flowering and seed formation in lentil (Lens culinaris Medik.)”  published in the October  issue, 2012 of In Vitro Cellular & Developmental Biology,  the authors, RH Sarker, Subrata Das and MI Hoque  at Botany Dept., DU have described a novel technique by which they succeeded in obtaining viable seeds without the intervention of rooted plants.   Healthy adventitious multiple shoots were produced when  explants of cotyledonary node and cotyledon attached decapitated embryos from two microsperma varieties (BM-1 and BM-4) of lentil (Lens culinaris Medik.) were cultured on MS medium containing 2.22 µM 6-benzylaminopurine (BA), 2.32 µM kinetin,  0.29 µM gibberellic acid , and 30.35 µM tyrosine. In vitro flowering was achieved from 74% of the regenerated shoots when cultured on MS medium supplemented with synthetic auxins  (indole-3-butyric acid, indole-3-acetic acid, and 1-naphthaleneacetic acid). The best response was obtained when shoots were regenerated on half-strength MS medium supplemented with 98.4 µM IBA and 2.69 µM NAA. Study with fluorescence microscopy revealed that 80% of the pollen grains obtained from in vitro grown flowers were viable. Following self-pollination typical growth of the pollen tube was observed within the stylar tissue. Most of these in vitro flowers developed pods within 10 to 12 days after flowering. Mature seeds of these pods germinated and produced healthy seedlings which were successfully transplanted into soil.

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