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Isolation of a lectin gene ; the protein it produces is harmul against pests

Isolation of a lectin gene ; the protein it produces is harmul against pests

In the April issue of the Indian J. Biotech 134-141 an, article under the caption, Isolation of lectin gene and development of resistant Nicotiana tabacum L. against Spodoptera litura was published. In that article Singh, Rashmi and six other associates have been  reported to have isolated a plant insecticidal protein known to be offering defense against insects. This protein either adversely affects the survivability and/or reduces the productiveness of the insects. Attack of insect pests causes a major loss of agricultural produce. In the present study, moth bean cDNA library was constructed for isolating a lectin gene. Using a binary pBinAR vector the authors isolated moth bean lectin (MbL) gene and introduced it into tobacco plants by Agrobacterium-mediated transformation method. The incorporation of transgene into the transformed tobacco was confirmed through molecular analysis. Insect bioassay of transgenic plants was done under laboratory conditions against Sporodoptera litura. The researchers observed an overall 50% reduction in the larval weight.

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