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RAPD-SCAR: Simple molecular technique for the genetic characterization, identification and authentication of organisms

RAPD-SCAR: Simple molecular technique for the genetic characterization, identification and authentication of organisms

Random amplified polymorphic DNA (RAPD) analysis is a popular and widely recognized molecular marker technique. RAPD is characterized by fewer requirements on template DNA, without any hazardous contamination, and at the same time it is also inexpensive. But there are some disadvantages, like poor reproducibility and low production. By prolonging the RAMP time from annealing to extension, the resolution and production of RAPD are greatly increased. We have established this improved RAPD for the genetic characterization recently. By the molecular cloning of RAPD fragments, sequence-characterized amplified regions (SCAR) can be developed, which can specifically identify species and even strains. Last year our lab has genetically characterized some medicinal plants like Gingko biloba [http://www.sciencedirect.com/science/article/pii/S0305197814002609], Lonicera japonica/ Japanese honeysuckle [http://link.springer.com/article/10.1007%2Fs11033-013-2703-3], Ganoderma lucidum/ Lingzhi or Reishi mushroom [http://www.sciencedirect.com/science/article/pii/S0305197814001379], Litchi chinensis/ Litchi [http://link.springer.com/article/10.1007/s11033-014-3755-8], Dimocarpus longan/ Logan fruit [http://www.geneticsmr.com/articles/2963], Gardenia jasminoides/ a garden plant,  and  Angelica sinensis [papers in press].

Later, we have developed species-specific or cultivar-specific SCAR markers for the identification of Lonicera japonica [http://revistas.ucr.ac.cr/index.php/rbt/article/view/13493/15531],  Dimocarpus longan [http://www.springerplus.com/content/2/1/501], and several Ganoderma and Litchi species [papers in press]. These are not very high-quality works, rather simple but interesting, and also important for the genetic preservation and conservation of plant species on the verge of extinction. Genetic characterization, making genetic map, and development of molecular markers for plants might be important and interesting for the genetic preservation and conservation of plants in BD and elsewhere all over the world. The improved RAPD, combined with ISSR and SCAR might be an easily maneuverable technique for any Bangladesh lab. Reported by Md. Asaduzzaman Khan, PhD asadkhanbmj@yahoo.com

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