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TALENs – A Novel Method to induce targeted somatic mutagenesis with 96% accuracy

TALENs – A Novel Method to induce targeted somatic mutagenesis with 96% accuracy

In a PNAS article, “Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs)”  published on 24th October, ’12, Ignor B. Dawid at the National Institute of Health, Bethesda, MD and nine  associates affiliated to various universities of Hong Kong, have described an approach for gene disruption at a particular site in various animal models such as Xenopus frog. In the above article they have described the procedure in which they induced  sexually transmittable somatic mutations in the above animal with a high degree of efficiency.  Furthermore they constructed  eight pairs of TALENs to target eight Xenopus genes all of which showed indel (an insertion or deletion) mutations with 95.7% of efficiency at the targeted loci.  The induced mutations were faithfully transmitted to all F1  progeny. Based on simple and reliable PCR-based approaches, the authors concluded that TALENs are an effective tool for targeted gene editing/knockout in Xenopus.

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