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Testing the fidelity of Plantlet regenarants in Jatropha curcas L

Testing the fidelity of Plantlet regenarants in Jatropha curcas L

In an article, Plantlet regeneration in Jatropha curcas L. and evaluation of genetic fidelity through RAPD markers published in the July issue of Indian J. Biotechnology (280-287), SB Maharana  and his four associates found that nodal explants of J. curcas were the best and that higher shoot bud formation took place in MS medium fortified with 8 µM N6-benzyladenine compared to 4.0 µM Kn as a supplement.  Regeneration  rate was good accounting for 80+4 established plantlets. It goes to the credit of the authors that using RAPD technique  they tested the genetic fidelity of the regenerants. Out of 40 decamers they used in their variation study,   they  observed that 14 primers  responded for amplification. The amplification pattern confirmed the genetic uniformity of the regenerants, thereby establishing the suitability of this protocol for in vitro micropropagation of J. curcas. It’s recommended that in order to ensure that regenerants  do not contain any variants, the somaclonal population should always be tested in order to ensure the uniformity of the plant population in the succeeding generations.

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