Dhaka March 6, ’14. In an article entitled, “Gemini virus Vectors Deliver Reagents for Plant Genome Engineering,” published online  followed by its publication in the January, ‘14 issue  of the Plant Cell (vol.26, 1-2), the authors report of many novel tools  for carrying out genome engineering work. It  may be mentioned here, that in several earlier  works, selected strains of Agrobacterium tumefaciens were used. The results of recent research  show that engineered nucleases can generate site-specific double-stranded DNA breaks. Furthermore, it has been shown that breaks can be repaired by nonhomologous end joining to create large or small deletions.  By homology-dependent repair specific sequence changes may be brought about using a template.